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Novartis human il 2 rhil 2
Human Il 2 Rhil 2, supplied by Novartis, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+il+2+rhil+2/2+human+il/pm42132875-357-75-79
Average 86 stars, based on 1 article reviews
human il 2 rhil 2 - by Bioz Stars, 2026-09
86/100 stars

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Produced:

Article Title: Microenvironmental Th9 and Th17 lymphocytes induce metastatic spreading in lung cancer
Article Snippet: .. The cells were primed with plate-bound anti-CD3 mAb (3 μg/mL, 145-2C11) and anti-CD28 mAb (5 μg/mL, 37.51), both mAbs produced and purified in-house) in the presence of recombinant human IL-2 (rhIL-2) (50 U/mL, Novartis), anti–mouse Biosciences) to obtain naive CD44–CD62L+CD4+ T cells using anti– CD4-V450 (RM4-5, BD Biosciences), anti–CD44-PE (IM7, eBioscience), and anti–CD62L-Alexa Fluor 700 (MEL-14, BD Pharmingen) mAbs in a dilution of 1:500. .. Sorting purity was maintained typically 3 5 7 1jci.org Volume 130 Number 7 July 2020 IL-17) using a BrdU colorimetric cell proliferation ELISA (Roche).

Purification:

Article Title: Microenvironmental Th9 and Th17 lymphocytes induce metastatic spreading in lung cancer
Article Snippet: .. The cells were primed with plate-bound anti-CD3 mAb (3 μg/mL, 145-2C11) and anti-CD28 mAb (5 μg/mL, 37.51), both mAbs produced and purified in-house) in the presence of recombinant human IL-2 (rhIL-2) (50 U/mL, Novartis), anti–mouse Biosciences) to obtain naive CD44–CD62L+CD4+ T cells using anti– CD4-V450 (RM4-5, BD Biosciences), anti–CD44-PE (IM7, eBioscience), and anti–CD62L-Alexa Fluor 700 (MEL-14, BD Pharmingen) mAbs in a dilution of 1:500. .. Sorting purity was maintained typically 3 5 7 1jci.org Volume 130 Number 7 July 2020 IL-17) using a BrdU colorimetric cell proliferation ELISA (Roche).

Recombinant:

Article Title: Microenvironmental Th9 and Th17 lymphocytes induce metastatic spreading in lung cancer
Article Snippet: .. The cells were primed with plate-bound anti-CD3 mAb (3 μg/mL, 145-2C11) and anti-CD28 mAb (5 μg/mL, 37.51), both mAbs produced and purified in-house) in the presence of recombinant human IL-2 (rhIL-2) (50 U/mL, Novartis), anti–mouse Biosciences) to obtain naive CD44–CD62L+CD4+ T cells using anti– CD4-V450 (RM4-5, BD Biosciences), anti–CD44-PE (IM7, eBioscience), and anti–CD62L-Alexa Fluor 700 (MEL-14, BD Pharmingen) mAbs in a dilution of 1:500. .. Sorting purity was maintained typically 3 5 7 1jci.org Volume 130 Number 7 July 2020 IL-17) using a BrdU colorimetric cell proliferation ELISA (Roche).

Article Title: Droplet microfluidic profiling of NK cell cytotoxicity with machine learning-enabled target-cell death analysis.
Article Snippet: .. For exNK cells, pbNK cells were expanded by co-culture for 3 weeks with irradiated (100 Gy) K562-mbIL-21 feeder cells (Clone 9, gift from Dr. Dean A. Lee's lab), replenished weekly (days 0, 7, 14) at a 1 : 2 NK : feeder ratio, maintaining NK density at 5 × 105 cells per mL post-replenishment, with media changes every 2–3 days (max 72 h) in NK culture medium (as described above) containing 50 ng mL−1 recombinant human IL-2 (rhIL-2) (Proleukin®, Novartis/Clinigen), at 37 °C and 5% CO2 in T25/T75 flasks. exNK-asc cells were obtained by culturing exNK in patient ascites for an additional 3 days. .. All blood draws, NK cell isolation, culturing, and preparation were performed by the Ashkar Lab (McMaster University, Hamilton, ON, Canada).

Irradiation:

Article Title: Droplet microfluidic profiling of NK cell cytotoxicity with machine learning-enabled target-cell death analysis.
Article Snippet: .. For exNK cells, pbNK cells were expanded by co-culture for 3 weeks with irradiated (100 Gy) K562-mbIL-21 feeder cells (Clone 9, gift from Dr. Dean A. Lee's lab), replenished weekly (days 0, 7, 14) at a 1 : 2 NK : feeder ratio, maintaining NK density at 5 × 105 cells per mL post-replenishment, with media changes every 2–3 days (max 72 h) in NK culture medium (as described above) containing 50 ng mL−1 recombinant human IL-2 (rhIL-2) (Proleukin®, Novartis/Clinigen), at 37 °C and 5% CO2 in T25/T75 flasks. exNK-asc cells were obtained by culturing exNK in patient ascites for an additional 3 days. .. All blood draws, NK cell isolation, culturing, and preparation were performed by the Ashkar Lab (McMaster University, Hamilton, ON, Canada).



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